Gram Stain Procedure Pdf Download, anti black magic bill pdf download
a0b7112eab Reporting Gram smears The report should include the following information: Staphylococcus in Gram Stain Numbers of bacteria present, whether many,moderate, few, or scanty Gram reaction of the bacteria, whether Grampositive or Gram negative Morphology of the bacteria, whether cocci, diplococci,streptococci, rods, or coccobacilli. Wash slide in a gentle and indirect stream of tap water for 2 seconds. Crystal violet stains all cells blue/purple Application of Mordant: The iodine solution (mordant) is added to form a crystal violet iodine (CV-I) complex; all cells continue to appear blue. (# Methanol fixation preserves the morphology of host cells, as well as bacteria, and is especially useful for examining bloody specimen material). Wait 10-15 seconds or add drop by drop to slide until decolorizing agent running from the slide runs clear . Gram-negative cells have thin layers of peptidoglycan, one to three layers deep with a slightly different structure than the peptidoglycan of gram-positive cells. Nitrate Reduction Test: Principle, Procedure and Results Leucine Amino peptidase (LAP) Test: Principle, Procedure and results 22 thoughts on “Gram Staining: Principle, Procedure and Results” Anonymous March 30, 2015 at 12:15 pm - Edit Reply The Note Given Is So Good!But Would You Mind Giving Additional Notes About Bacterial Growth And Its Classification? hudu said May 19, 2015 at 8:18 pm - Edit Reply I like it Ranishree August 31, 2015 at 10:51 pm - Edit Reply Nice explanation.its really helpful during viva in exam Jean September 17, 2015 at 3:58 pm - Edit Reply Very good description Anonymous September 29, 2015 at 8:26 am - Edit Reply Am honored for the information Tim Timothyrhyz October 8, 2015 at 12:21 pm - Edit Reply I love the GrAm staining,its precise but resourceful,thanks ISRAEL MUDOMBO October 12, 2015 at 1:53 pm - Edit Reply Nice explanations on Gram staining, will help in coming exams and assignments.
The decolorizing agent, (ethanol or an ethanol and acetone solution), interacts with the lipids of the membranes of both gram-positive and gram negative bacteria. The highly cross-linked and multi-layered peptidoglycan of the gram-positive cell is dehydrated by the addition of ethanol. Results: Gram-negative bacteria will stain pink/red and Gram-positive bacteria will stain blue/purple. After decolorization, the gram-positive cell remains purple in color, whereas the gram-negative cell loses the purple color and is only revealed when the counterstain, the positively charged dye safranin, is added. With ethanol treatment, gram-negative cell walls become leaky and allow the large CV-I complexes to be washed from the cell. In aqueous solutions crystal violet dissociates into CV+ and Cl ions that penetrate through the wall and membrane of both gram-positive and gram-negative cells. Variations in Gram Staining Results Various factors influence the results of Gram staining. Application of counter stain (safranin): The red dye safranin stains the decolorized gram-negative cells red/pink; the gram-positive bacteria remain blue. Observe the results of the staining procedure under oil immersion (100x) using a Bright field microscope. Cancel reply Follow me on:Subscribe to Microbeonline via Email Enter your email address to subscribe to this blog and receive notifications of new posts by email.
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